These data may indicate Treg activation in the lymphoid organs prior to homing to the TME, as has been previously suggested.54 However, despite previous reports on a potential role for CCR8 in the recruitment,54 activation and/or suppressive capacity55 of Tregs, we observed that a lack of CCR8 expression or a Nb-mediated blockade of CCR8 signaling did not affect these Treg characteristics, nor did it affect tumor growth. The gene discussed is CCR8; the disease is neoplasm.