To obtain an idea about the expression pattern of Ccr8 in the TME, Ccr8 expression was first assessed via qRT-PCR within the CD45+ hematopoietic and CD45− non-hematopoietic fraction of subcutaneously grown Lewis Lung Carcinoma (LLC)-OVA, B16-OVA melanoma and MC38 colon carcinoma tumors. This evidence concerns the gene CCR8 and melanoma.