This analysis confirmed the NanoString expression data and showed that tumor-infiltrating human lymphocytes expressed high levels of PD-1, T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) and lymphocyte-activation gene-3 (LAG-3, figure 7B–E). This evidence concerns the gene PDCD1 and neoplasm.