FBL and amyotrophic lateral sclerosis: We measured nucleolar, nuclear and cytoplasmic areas using fibrillarin immunofluorescence, DAPI immunofluorescence and background fluorescence respectively in lumbosacral spinal motor neurons (SMNs) from 11 SALS, C9-ALS and control nervous systems each (Table 1, Fig. 1a and Additional file 2: Fig. S2a).