We next knocked down LATS2 (the knockdown efficiency is shown in Fig. 6d and Fig. S5f) in 5’tiRNA-His-GTG-inhibited CRC cells and performed CCK8, colony formation, and apoptosis assays to investigate whether 5’tiRNA-His-GTG plays an oncogenic role in CRC progression dependent on LATS2. This evidence concerns the gene LATS2 and colorectal carcinoma.