To investigate the biological functions of LINC00342 in CRC cells, specific shRNA was used to knock down LINC00342 expression in SW480 and HT-29 cells, and real-time PCR (Fig. 2a) and green fluorescence microscopy (Fig. 2b) were performed to confirm the transfection efficiency. The gene discussed is LINC00342; the disease is colorectal carcinoma.