To validate the cellular effect of Rsu1 on F-actin bundling, we overexpressed different Rsu1 variants in HeLa cells, the immortal human cervical cancer cells, and found that the wild-type Rsu1 localized to the FA while the PINCH1-binding deficient mutants (Y140K and DY/KK) failed to accumulate at the FA (Figure 5A). Here, LIMS1 is linked to cervical cancer.