ABCB7 and cancer: Following successful demonstration of transient HSATII and α-sat expression in HeLa cells, stably transfected cell lines were generated for both HeLa and a primary (non-transformed) human fibroblast cell line to ensure that the results observed were not simply an effect of expressing satellite RNA in cancer cells, in which the nuclear and chromatin environment can be drastically different from normal cells (Carone and Lawrence, 2013; Zink et al., 2019).