•   Appearance*•   Total leukocytes*•   Total neutrophils*•   Total lymphocytes*•   Protein*•   Glucose*•   Gram stain*•   Adenosine deaminase activity*•   Bacterial culture•   India ink stain*•   Cryptococcal antigen* and culture•   Microscopy for acid-fast bacilli•   Mycobacterial culture•   NAAT forMycobacterium tuberculosis•   NAAT for any virus•   Syphilis serology*•   Any other test informing an alternativediagnosis. Here, ADA is linked to syphilis.