SOCS3 and persistent truncus arteriosus: Four weeks after TAC operation, echocardiographic assessment revealed that TAC significantly impaired contractile function, as reflected by decreased ejection fraction (EF%) and fractional shortening (FS%) in rAAV9-ZsGreen-injected mice compared with Sham groups, whereas rAAV9-SOCS3-injected mice recovered cardiac dysfunction similar to or better than Sham mice (Figure 2A).