TNF and central nervous system cancer: A two-fold increase in NO synthesis (2.2 μM) was observed in glioma cells treated with inflammatory stimulus, a combination of 100 ng/ml of TNF and 5 μg/ml of LPS, in the presence of 20 μg/ml of CuHARS compared to the cells treated with the inflammatory stimulus alone (1.1 μM), 48 h after the treatment (Figure 4A).