An interesting observation in the experiment is that the NO yield at 50 μM CysNO for BMVECs is higher than that observed for glioma cells (Figure 3A), hence indicating that glioma cells can produce NO at reduced level compared to the endothelial cells which are capable of constitutive NO synthesis due to the presence of endothelial nitric oxide synthase (eNOS) that can contribute to the major part of NO production in the vascular system (Zhao et al., 2015). This evidence concerns the gene NOS3 and glioma.