Applying the ibidi wound scratch apparatus we found that exogenous addition of Hpa2 attenuates the migration of 5637 (Figure 4A, upper vs middle panels), primary MCF10A human breast (Supplementary Figure 1C), primary porcine endothelial (PAEC; Supplementary Figure 1D, upper panels), head and neck carcinoma SINH-013 (Supplementary Figure 1D, middle panel), and lung carcinoma A549 (Supplementary Figure 1D, lower panel) cells. Here, HPSE2 is linked to head and neck carcinoma.