The results suggested a surprising degree of consistency, CD274 (in 32 tumor types, except THYM, Supplement Figure 5), CD8A (in 32 tumor types, except UCS, Supplement Figure 6), GZMA (in 33 tumor types, Supplement Figure 7), and PRF1 (in 33 tumor types, Supplement Figure 8) were all positively correlated with the stromal score or immune score in almost all 33 tumor types, which gave us the basis for continued classification of samples according to the IRG characteristics. This evidence concerns the gene PRF1 and neoplasm.