To this purpose, a RIZtot mRNA interference experiment was set up in MCF-7 and MDA-MB-231 breast cancer cell lines with a siRNA spanning a sequence coding for a region common to both RIZ1 and RIZ2 gene products (aa 333–340 of RIZ1 protein or aa 132–139 of RIZ2 protein), indicated as siRNA-total (see also Figure S1). Here, PRDM2 is linked to breast cancer.