It seems that Cholesterol biosynthesis pathway is required for HCC development.In a proteomics study on hepatitis B leading to hepatocellular carcinoma, Gao et al.introduced the biomarkers of PYCR2 and ADH1A for metabolic programming in proteomic subgroups.They revealed CTNNB1 and TP53 mutation associated signaling and metabolic profiles withHC (70). The gene discussed is PYCR2; the disease is hepatocellular carcinoma.