Expression of ZAK-LF transformed immortalised murine fibroblasts and induced xenograft tumour formation in immunodeficient mice, while depletion of ZAK-LF reduced the growth of PRPF6-overexpressing colon cancer cells in vitro and in xenografts (Adler et al., 2014; Lokody, 2014). This evidence concerns the gene MAP3K20 and colonic neoplasm.