As the initial live offspring produced by GST would be heterozygote due to modification of the male germline only, we chose to evaluate gene editing at three biomedically relevant loci associated with either dominant forms of diabetes mellitus, Hepatocyte nuclear factor-1 alpha (HNF1a) and insulin (INS), or a common safe-harbor locus for transgene insertion, ROSA26 (Figure 2A). Here, HNF1A is linked to diabetes mellitus.