PRF1 and neoplasm: To further verify the levels of immune activation in different immune subtypes, we examined the expression levels of common effectors of immunity, such as granzyme A (GZMA), granzyme B (GZMB), and perforin (PRF1) (Figure 1B; Mandal et al., 2016) and the immune cytolytic activity (CYT, an indicator of tumor local immunity, Figure 1C; Rooney et al., 2015).