After knockdown of FOXO1, apoptosis assay indicated that silenced FOXO1 did not significantly affect the later apoptosis of MDS-L cells, but significantly decreased early apoptosis of MDS-L cells, suggesting that FOXO 1 activation is involved mostly in the early stages of DAC-induced apoptosis (Figure 3E). This evidence concerns the gene FOXO1 and myelodysplastic syndrome.