CCR6, CXCR3, CXCR6, and CCL5 were the genes in one significant module, and they were enriched in chemokine receptors bind chemokines, chemokine-mediated signaling pathway, etc. In peripheral blood, CD4+ TEMRA/TEFF and CD8+TCM showed few DEGs, while CD4+ TN and CD8+ TEMRA/TEFF showed more DEGs between colon cancer and rectal cancer. This evidence concerns the gene CCR6 and malignant colon neoplasm.