One of the limitations in these clinical trials is the lack of the comparison of tumor samples before and after DC treatment, but in the mouse model, such change of ovarian cancer microenvironment has been illustrated, DC vaccination promotes the proliferation of CD4+T cells and CD8+T cells and decreases the level of MDSCs, Tregs and tumor-associated macrophages (90). This evidence concerns the gene CD8A and ovarian carcinoma.