MAP1LC3B and Parkinson disease: As our previous study has revealed autophagic flux dysfunction in 6-OHDA-induced PD model, which contributed to the neuron death (He et al., 2018), and the in vivo results above could not fully reveal the actual state of autophagic flux upon ghrelin treatment, we further applied adenovirus Ad-mCherry-GFP-LC3B to assess the effect of ghrelin on autophagic flux in the 6-OHDA-induced SH-SY5Y cell model.