Acute HFD treatment (24 h) in mice induced increased GFAP and S100B expression in the mediobasal hypothalamus (MBH) accompanied by hyperphagia, suggesting a potential role of activated astrocytes in mediating energy intake and that astrocyte activation arises before obesity (Buckman et al., 2015). Here, GFAP is linked to obesity due to melanocortin 4 receptor deficiency.