In agreement of the aforementioned recent study observations by Dusart et al. [15], our study found a significantly lower serum Nestin values in breast cancer group compared to the control group, with a cut-off of 39.9 pg/mL representing the best value for discriminating patients with breast cancer from normal women, where, lower Nestin values were found to be associated with increased probability to cancer. The gene discussed is NES; the disease is breast cancer.