SHBG (β = 0.311, P < 0.001), AD (β = 0.834, P < 0.001) and BMI (β = 0.161, P = 0.024) were factors influencing TT in PCOS patients, whereas SHBG (β = 0.256, P = 0.005) and AD (β = 0.868, P < 0.001) were factors influencing TT in healthy volunteers. Here, SHBG is linked to polycystic ovary syndrome.