Thus, this study aimed to: (1) evaluate HRQoL in PLWHA receiving ART; (2) determine the sensitivity of the EuroQol questionnaire to differentiate the HRQoL in different stages of HIV infection, CD4 T lymphocytes, and VL and; (3) identify factors influencing HRQoL in Pakistani patients with diverse cultural background. This evidence concerns the gene CD4 and HIV infectious disease.