Primary, metastatic or recurrent tumours were enzymatically digested and grown in Matrigel using a medium containing Rspondin-1, Noggin, EGF, FGF2 and 10, nicotinamide, PGE2 and inhibitors of TGF-β and p38 MAPK signalling (Table 1). The gene discussed is TGFB1; the disease is neoplasm.