Furthermore, using GEPIA database, we found that high expression of S100A family members (eg S100A6, S100A10, S100A11, S100A14 and S100A16) significantly associated with low expression of the marker genes from TILs (eg CD8A, CD8B, CD2, CD3D and CD3E) in PC (Table 3). The gene discussed is S100A16; the disease is pachyonychia congenita.