Among the 20 samples tested, 18 (90%), 20 (100%), 20 (100%), 19 (95%), 14 (70%), and 20 (100%) were PD-L1-positive for squamous cell carcinoma, nasal adenocarcinoma, transitional cell carcinoma, anal sac gland carcinoma, soft tissue sarcoma, and osteosarcoma, respectively. The gene discussed is CD274; the disease is squamous cell carcinoma.