All were negative for mutations in the major PD genes and other genes associated with neurodegenerative brain diseases (Additional file 1: Table S5), except for the PD patient P9 who carried the LRRK2 p.Arg1441Cys pathogenic mutation besides the VPS13C p.Ile2789Thr/p.Ile3726Val un-phased alleles. This evidence concerns the gene LRRK2 and Parkinson disease.