Mechanistically, the PD-L1 overexpression and downregulation vectors were successfully delivered into CR-GC cells (P < 0.05, Fig. 5a, b), and we verified that PD-L1 negatively regulated NLRP3 in CR-GC cells (P < 0.05, Fig. 5a, b). The gene discussed is NLRP3; the disease is gastric cancer.