Combined labeling of three primary BBB components, endothelium, basement membrane and astrocytes or glioma cells (Fig. 5a and b) and detailed high-resolution 3D reconstruction of normal CTX and GBM (Fig. 5c and d), depicted complex, multilayered fenestration in GBM compared to the regular and continuous structure in normal CTX with clear variations in GFAP layers (Fig. 5c–e). Here, GFAP is linked to glioma.