Finally, based on data from the GEO, TCGA, and HPA databases and our clinical samples, we found that compared with normal tissues, the TIMP1 protein was expressed highly and significantly in COAD samples, while the ITLN1, TSPAN11, CPRC5B, and CXCL13 proteins were poorly expressed in colon cancer samples. This evidence concerns the gene TIMP1 and malignant colon neoplasm.