We provide a comprehensive set of evidence revealing upregulation of TMEM45B and NANS in PC initiation (Figure 3A), progression to mPCs (Figure 3B), their association with TMPRSS2-ERG fusion (Figure 4), progression to CRPC (Figure 6), and their biomarker value in predicting PC recurrence in two-independent datasets (Figure 7). Here, NANS is linked to pachyonychia congenita.