The increase of stroma and vimentin-positive stromal cells of Cluster B GBCs indicates that fibroblasts in the stroma are induced to CAFs by various crosstalk between cancer cells and stromal cells in TME, including inflammation and the TGF-β and EMT pathways; then, this crosstalk in TME induces cancer aggressiveness and worse prognosis [39]. The gene discussed is TGFB1; the disease is cancer.