To investigate if melanoma cells are capable of recruiting PMN-MDSC via CXCR2 from the periphery, we cultured Ret melanoma cells in the bottom compartment of a transwell and determined the number of migrated PMN-MDSC towards Ret cells through SVEC4-10 lymphatic endothelial cells (Figure 4E). The gene discussed is CXCR2; the disease is melanoma.