Therefore, based on the above information, which suggest an important functional role for MACROD1 in skeletal muscles in cells and a link between MACROD2 and various neurological conditions, we decided to understand the physiological role of MACROD1 and MACROD2 hydrolases by subjecting knockout mice to a phenotyping battery to assess multiple, spontaneous aspects of mouse behaviour including locomotor activity, anxiety, motor coordination, grip strength, short-term memory and attention. This evidence concerns the gene MACROD2 and Anxiety.