Furthermore, lysates of Lactobacillus acidophilus also have enhanced anti-tumor efficacy of anti-CTLA-4 (cytotoxic T lymphocyte-associated protein 4) blocking antibody in mice CRC models, associated with increased CD8+ T cells, decreased numbers of T regulatory cells (Tregs), and decreased M2 macrophages in the tumor microenvironment [22]. The gene discussed is CTLA4; the disease is colorectal carcinoma.