As we know, whole exome gene sequencing provides a diagnosis in 25–29% of individuals with disorders suggestive of a genetic etiology in pediatric and adult populations[19] and it is a first-tier clinical diagnostic test for individuals with neurodevelopmental disorders.[20] Therefore, in order to confirm the SRY gene in the diagnosis of testicular DSD, the accuracy of whole exome gene sequencing is markedly better than PCR. This evidence concerns the gene SRY and disorder of sexual differentiation.