RNA-seq revealed that KCs from NASH livers expressed lower amounts of KC specific genes such as Timd4, Pcolce2, and Arg2. Timd4 and Arg2 were also decreased in KCs lacking LXRα knockout, suggesting that loss of LXR activity at their enhancers results in deactivation in the NASH environment (21). This evidence concerns the gene PCOLCE2 and metabolic dysfunction-associated steatohepatitis.