The SPANX-A/D subfamily was highly expressed in A375, MelHO and Colo-800 melanoma cell lines (Fig. 1A), as well as in several cell lines derived from distinct tumours, such as SW480 and HCT-8 (colorectal adenocarcinoma), HeLa (epithelioid cervix carcinoma), A2780 (ovary adenocarcinoma), SMS-KCNR (neuroblastoma cell line), and MCF-7 (mammary adenocarcinoma) (Supplementary Fig. S1). This evidence concerns the gene SPANXA1 and colorectal adenocarcinoma.