More importantly, ARHGEF12 expression was also positively associated with immune-related genes, such as CD44, CD47, CD53, CD59, and CD274. Therefore, we suspected that, like its related genes, ARHGEF12 was crucial in the Rho signaling pathway and might be related to diffuse large B cell lymphoma initiation, proliferation, metastasis and treatment, but the deep biological basis of these relationships needs further study. This evidence concerns the gene CD53 and diffuse large B-cell lymphoma.