To investigate whether the activation status of pDCs in SSc is associated with the aberrant expression of miR-126 and miR-139-5p, we analyzed their expression after the activation of healthy pDCs with TLR7/8 and TLR9 ligands (R848 and CpG-C, respectively) for 24 h. This evidence concerns the gene TLR7 and systemic sclerosis.