To investigate whether the stemness-high human colorectal cancer (CRC) cells could induce a stronger angiogenesis of endothelial cells (ECs), we first prepared the conditioned media (CM) from the vector-control as well as the stemness-high GATA6-overexpressing clones established previously from HCT-116 (116 Vec, OE4, and OE6) and HT-29 (29 Vec, OEC, and OED) human CRC cells [43] whose GATA6 levels were 4- and 5-folds higher than their respective control clones (Figure S1), by collecting the media containing 2% FBS used to culture these cells for 48 h. This evidence concerns the gene GATA6 and colorectal cancer.