To further investigate whether the GATA6-overexpressing human CRC cells could promote angiogenesis in vivo, immunohistochemical (IHC) staining was performed using tissue samples resected from the tumors grown from the clones as well as their parental counterparts transplanted subcutaneously into nude mice by using antibodies against CD31 and endomucin, two EC markers, as probes, respectively. This evidence concerns the gene PECAM1 and colorectal carcinoma.