After assuring that the stemness properties of these clones were not significantly affected by a short-term incubation in low serum condition (Figure 1A), we treated HUVECs with CM collected from the clones and found that CM collected from the GATA6-overexpressing human CRC clones induced marked increases of the tube formation, migration, invasion and even DNA synthesis in HUVECs (Figure 1B–E). This evidence concerns the gene GATA6 and colorectal carcinoma.