To test IL-10 effects on the production of most important pro-inflammatory cytokines, interleukin-6 (IL-6), tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β), splenocytes from NZB/W F1 animals with beginning nephritis were stimulated for 48 h with a mix of lipopolysaccharide (LPS) and Pam3CSK4 in the presence or absence of α-IL-10. The gene discussed is IL10; the disease is nephritis.