These organoids developed CDKN2A−/−/CDKN2B−/−/EGFROE/EGFRvIIIOE, NF1−/−/PTEN−/−/TP53−/−, and EGFRvIIIOE/CDKN2A−/−/PTEN−/− genotypes, which are commonly found in glioblastoma. Here, TP53 is linked to glioblastoma.