PTK6 knockdown resulted in a decrease in E-cadherin and ZO-1 epithelial markers and an increase in vimentin, ZEB1, and claudin-1 mesenchymal markers via STAT3-MEK5/ERK5 in colon cancer cells, which was reversed by PTK6 reintroduction. Here, MAP2K5 is linked to malignant colon neoplasm.