Methylation levels of markers GHSR, SST, and ZIC1 were significantly higher in both natural voided urine (all, Mann–Whitney U test: p < 0.001) and catheter urine (Mann–Whitney U test: p < 0.001, p = 0.002, and p = 0.034, respectively) of cervical cancer patients as compared to the natural voided urine of age-matched controls. Here, GHSR is linked to cervical carcinoma.