TNF and lung cancer: To further investigate the underlying mechanism, we first examined the phosphorylation status of LATS at hydrophobic motif, which is essential for LATS kinase activity using anti-phospho LATS1-T1079/LATS2-T104 antibody in TNF-stimulated HEK293A (Fig. 1A), NIH3T3 (Fig. 1B), and lung cancer cell A549 (Fig. S1A).