SERPINA3 and breast cancer: To further elucidate the effect of SERPINA3 on BC growth, we analyzed the cell cycle distributions of SERPINA3-overexpression (SERPINA3-OE) in BT-549, MDA-MB-231 and MDA-MB-436 cells by flow cytometry analysis, and the results indicated that cell proliferation was blocked at G1 in the experimental group and that the number of cells in S phase increased (Fig. 2i, j).