We selected this cell line considering the role of monocytes in modulating the epithelial barrier function and cytokine secretion in a CeD-specific context (Delbue et al., 2019), as well as for the technical ability to alter the expression of TRAFD1 using siRNA and the quick response of monocytes to LPS (Sharif et al., 2007). The gene discussed is TRAFD1; the disease is cranioectodermal dysplasia.